منابع مشابه
Cytochemical Localization of Catalase in Leaf Microbodies (peroxisomes)
Segments of mature tobacco leaves were fixed in glutaraldehyde, incubated in medium containing 3,3'-diaminobenzidine (DAB) and hydrogen peroxide, and postfixed in osmium tetroxide. Electron microscopic observation of treated tissues revealed pronounced deposition of a highly electron-opaque material in microbodies but not in other organelles. The coarsely granular reaction product is presumably...
متن کاملDual targeting of yeast catalase A to peroxisomes and mitochondria.
Yeast catalase A (Cta1p) contains two peroxisomal targeting signals (SSNSKF) localized at its C-terminus and within the N-terminal third of the protein, which both can target foreign proteins to peroxisomes. In the present study we demonstrated that Cta1p can also enter mitochondria, although the enzyme lacks a classical mitochondrial import sequence. Cta1p co-targeting was studied in a catalas...
متن کاملBiogenesis of peroxisomes: immunocytochemical investigation of peroxisomal membrane proteins in proliferating rat liver peroxisomes and in catalase-negative membrane loops
Treatment of rats with a new hypocholesterolemic drug BM 15766 induces proliferation of peroxisomes in pericentral regions of the liver lobule with distinct alterations of the peroxisomal membrane (Baumgart, E., K. Stegmeier, F. H. Schmidt, and H. D. Fahimi. 1987. Lab. Invest. 56:554-564). We have used ultrastructural cytochemistry in conjunction with immunoblotting and immunoelectron microscop...
متن کاملTHE EFFECT OF GLUTARALDEHYDE ON CATALASE Biochemical and Cytochemical Studies with Beef Liver Catalase and Rat Liver Peroxisomes
Peroxidatic activity of catalase (E .C .1 .11 .1 .6) was discovered by Keilin and Hartree (1), and it has been firmly established that, catalase oxidizes various substrates such as ethanol, methanol, nitrite, and formate (2-4) . Crystalline beef liver catalase, used as an enzyme tracer (5), and the peroxisomal catalase (6, 7) have been visualized cytochemically by a modification of the 3,3'diam...
متن کاملAbnormality in catalase import into peroxisomes leads to severe neurological disorder.
Peroxisomal disorders are lethal inherited diseases caused by either defects in peroxisome assembly or dysfunction of single or multiple enzymatic function(s). The peroxisomal matrix proteins are targeted to peroxisomes via the interaction of peroxisomal targeting signal sequences 1 and 2 (PTS1 or PTS2) with their respective cytosolic receptors. We have studied human skin fibroblast cell lines ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.m006347200